Watching ESBL reveal itself in real time 😀 Extended-spectrum β-lactamase (ESBL) producing bacteria can be detected using the Double Disc Synergy Test (DDST). In this video, an isolate was tested on Mueller–Hinton agar using: • AMC (Amoxicillin Clavulanic acid, 10 µg) • CRO (Ceftriaxone, 30 µg) • Other third-generation cephalosporins The AMC disc was placed at the center, with cephalosporins positioned 20 mm apart. During incubation at 37°C, notice the progressive extension of the inhibition zon