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You have a 5 kb vector fragment and run 5 µl on an agarose gel next to 5 µl of the same ladder we use in our lab manual. The total amount of DNA ladder loaded in 5 µl was 0.5 µg. The intensity of the 5 kb fragment band is similar to that of the 3 kb band in the ladder. What is the approximate concentration of the vector sample that you loaded in ng/µl?
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