Quantitative reverse transcription polymerase chain reaction, also called RT-qPCR, is used to detect and quantify RNA. Total RNA or mRNA is first transcribed into complementary DNA …

A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR).

RT-qPCR, or quantitative reverse transcription PCR, combines the effects of reverse transcription and quantitative PCR or real-time PCR to amplify and detect specific targets.

Dec 1, 2024 · Here, we provide an overview of RT-qPCR, focusing on essential reagents, the importance of primer design, the detailed workflow, and data analysis methods. This guide will …

Aug 23, 2025 · Reverse transcription quantitative polymerase chain reaction, commonly known as RT-qPCR, is a laboratory technique used to detect and measure specific ribonucleic acid …

Real-time PCR/qPCR assays have become the tool of choice for the rapid and sensitive determination and quantitation of nucleic acid in various biological samples, with diverse …

Reverse transcription quantitative PCR (RT-qPCR) has delivered significant insights in understanding the gene expression landscape. Thanks to its precision, sensitivity, flexibility, …

Nov 22, 2022 · RT-qPCR is one of the most commonly used tools in molecular biology labs to analyze and quantify nucleic acids in diverse biological samples quickly and accurately.

Combined RT-PCR and qPCR are routinely used for analysis of gene expression and quantification of viral RNA in research and clinical settings. The close association between RT …

qPCR can also be modified to detect and quantitate RNA by adding a reverse transcriptase step upstream of the qPCR assay to generate cDNA (i.e., RT-qPCR). Reverse transcription can be …