In the 1990s, NetFlow version 5 started out as a Cisco Systems proprietary protocol for capturing and sending information about network traffic flows to a collection point. NetFlow can be enabled on ...

Protein phosphorylation is a ubiquitous and key step in signaling pathways. For flow cytometry staining, the right protocol can help accurately capture these phosphorylated targets for clear-cut data.

Flow cytometry analysis 1. Analyze samples on a flow cytometer equipped with appropriate filters, corresponding to the selected fluorophore labels. 2. To achieve a CV of 1–10%, a minimum of 107–105 ...

Flow cytometry is an essential tool enabling precise cellular analysis across many fields, including immunology, oncology and cell biology research. However, suboptimal staining protocols can leave ...

High-throughput flow cytometry of adherent cells is difficult because the creation of single cell suspensions can damage cells and yield artificial results. We describe a protocol to increase the ...