With standard PCR systems, users are required to set a predefined quantity of cycles according to assay and sample-specific factors. Single-cell sequencing tests demand knowledge of the number of ...
With the increase in positive COVID tests, physicians, contact tracers, and hospitals in our town are very busy. I would imagine the same can be said for your community. With this time of year being ...
PCR cycle selection is a delicate balancing act. When preparing RNA-seq libraries, choosing too many cycles risks overcycling, which can compromise data quality and distort transcript representation.
COVID-19 is commonly diagnosed by quantitative fluorescence real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) (1, 2). The test is the sequential action of two enzymes–an ...