Nearly 100 years ago, a seemingly simple discovery revolutionized the microscope. The introduction of phase contrast, which garnered a Nobel Prize in 1953, brought into clear view structures inside ...
Over the past centuries, optical microscopy has been fundamental for exploring and understanding structural features and sample dynamics. Fluorescence microscopy, developed in the early 20th century, ...
In this review, we will look ahead and discuss potential future avenues of fluorescence imaging with light-sheets. We will describe how volumetric and temporal imaging barriers limit the application ...
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